Title :
Study on Methods of Protein Immobilization for AFM
Author :
Wang, Jianhua ; Wang, Congzhou
Author_Institution :
Coll. of Bioeng., Chongqing Univ., Chongqing, China
Abstract :
The potentialities of the atomic force microscopy (AFM) make it a tool of the undeniable value for the study of biomolecule samples. One of the main steps for the application of atomic force microscopy to the study of proteins has been the self-assembled monolayers (SAMs). The methods of physical adsorption and chemical modification are utilized to immobilize proteins to substrates efficiently. SAMs play an important role in protein immobilization. Membrane proteins need especial immobilization procedures in order to maintain their biological activities in vitro. This review proposes some fundamental methods of protein immobilization for AFM study.
Keywords :
adsorption; atomic force microscopy; biochemistry; biological techniques; biomembranes; monolayers; proteins; proteomics; self-assembly; AFM; atomic force microscopy; biological activities; biomolecule samples; chemical modification; membrane proteins; physical adsorption; protein immobilization; self-assembled monolayers; Atomic force microscopy; Atomic layer deposition; Biomembranes; Bovine; Chemicals; Coatings; Electrostatics; Proteins; Scanning electron microscopy; Substrates;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5514974
