Title :
Dielectrophoresis and Electrorotation of Spheroidal Cells after nsPEF Induced Electroporation
Author :
Hu, Qin ; Fadiran, Olaoluwa ; Li, Wenjun ; Joshi, Ravindra P.
Author_Institution :
Sch. of Eng. & Technol., Central Michigan Univ., Mount Pleasant, MI, USA
Abstract :
The impact of nanosecond, pulsed electric fields (nsPEF) induced biological membrane electroporation on the Clausius-Mossotti (CM) factor is studied. The electrorotational speed, which is proportional to the imaginary part of the CM factor, can be used for the noninvasive detection and monitoring of cellular membrane poration. Also, the dielectrophoresis (DEP) characteristics of normal and malignant white cells are different and one cell type can be more prone to be porated than another. These results point to the possibility of judiciously selecting electrical parameters for effective cell separation, especially in high conductivity media.
Keywords :
biochemistry; bioelectric phenomena; biological techniques; biomembranes; cellular biophysics; electrophoresis; Clausius-Mossotti factor; cell separation; cellular membrane poration; dielectrophoresis; electrical parameters; electrorotational speed; high conductivity media; malignant white cells; noninvasive detection; nsPEF induced electroporation; pulsed electric fields; spheroidal cells; Dielectrophoresis;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5514999
