Dielectrophoresis and Electrorotation of Spheroidal Cells after nsPEF Induced Electroporation

Author

Hu, Qin ; Fadiran, Olaoluwa ; Li, Wenjun ; Joshi, Ravindra P.

Author_Institution

Sch. of Eng. & Technol., Central Michigan Univ., Mount Pleasant, MI, USA

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

4

Abstract

The impact of nanosecond, pulsed electric fields (nsPEF) induced biological membrane electroporation on the Clausius-Mossotti (CM) factor is studied. The electrorotational speed, which is proportional to the imaginary part of the CM factor, can be used for the noninvasive detection and monitoring of cellular membrane poration. Also, the dielectrophoresis (DEP) characteristics of normal and malignant white cells are different and one cell type can be more prone to be porated than another. These results point to the possibility of judiciously selecting electrical parameters for effective cell separation, especially in high conductivity media.

Keywords

biochemistry; bioelectric phenomena; biological techniques; biomembranes; cellular biophysics; electrophoresis; Clausius-Mossotti factor; cell separation; cellular membrane poration; dielectrophoresis; electrical parameters; electrorotational speed; high conductivity media; malignant white cells; noninvasive detection; nsPEF induced electroporation; pulsed electric fields; spheroidal cells; Dielectrophoresis;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5514999

Filename

5514999