DocumentCode :
3092542
Title :
Characterization of microbubble-loaded stem cells for targeted cell therapy
Author :
Kokhuis, Tom J. A. ; Skachkov, I. ; Naaijkens, Benno ; Juffermans, Lynda J. M. ; Kamp, Otto ; van der Steen, Anton F. W. ; Versluis, Michel ; de Jong, Nico
Author_Institution :
Dept. of Biomed. Eng., Erasmus Med. Center, Rotterdam, Netherlands
fYear :
2013
fDate :
21-25 July 2013
Firstpage :
1146
Lastpage :
1149
Abstract :
The use of stem cells for the repair of damaged cardiac tissue after a myocardial infarction holds great promise. However, a common finding in experimental studies is that only a small fraction of the transplanted cells engraft in the infarcted area of the heart. In this study we investigated the use of stem cells conjugated with targeted microbubbles, creating echogenic complexes dubbed StemBells, to achieve increased stem cell delivery. The dynamics of the constructs in response to an ultrasound wave was characterized using high speed optical imaging. A modified Rayleigh-Plesset equation was developed to corroborate our experimental findings. We show that acoustic radiation force can be used to propel StemBells to the wall of a 200 μm vessel in a chicken embryo. This technique therefore facilitates the possibility to localize stem cell delivery to increase the engraftment and improve the clinical outcome of stem cell therapy.
Keywords :
biomedical optical imaging; blood vessels; bubbles; cardiovascular system; cellular transport; high-speed optical techniques; medical disorders; muscle; tissue engineering; ultrasonic therapy; StemBells; acoustic radiation force; clinical outcome; construct dynamics; damaged cardiac tissue repair; echogenic complexes; engraftment; heart infarcted area; high speed optical imaging; microbubble-loaded stem cell characterization; modified Rayleigh-Plesset equation; myocardial infarction; size 200 mum; stem cell delivery localization; stem cell therapy; targeted cell therapy; targeted microbubble; transplanted cells; ultrasound wave; vessel wall; Force; Mathematical model; Medical treatment; Myocardium; Resonant frequency; Stem cells; Ultrasonic imaging; Bjerknes force; acoustic radiation force; cardiac repair; stem cell therapy; targeted microbubble;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Ultrasonics Symposium (IUS), 2013 IEEE International
Conference_Location :
Prague
ISSN :
1948-5719
Print_ISBN :
978-1-4673-5684-8
Type :
conf
DOI :
10.1109/ULTSYM.2013.0293
Filename :
6724853
Link To Document :
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