Title :
Optimization of the Operational Parameters for Oestrone Quantification in Sewage and River Water Matrices Using Response Surface Method
Author :
Wan Qiaoling ; Yang Chun ; Zhang Zhi ; Lian Peng
Author_Institution :
Key Lab. of Three Gorges Reservoir Region´s Eco-Environ. under Minist. of Educ., Chongqing Univ., Chongqing, China
Abstract :
Response surface method (RSM) is a set of mathematical and statistical methods for experimental design and evaluating the effects of variables and searching optimum conditions of variables to predict targeted responses. Oestrone (E1) is one of the most important steroid estrogens in sewage and waterbody. An internal standard method of HPLC-MS-MS for E1 is established with its operational parameters in the multiple reaction monitoring (MRM) mode being optimized by RSM. With a sample pretreatment using two-step solid phase extraction (SPE), the detection limit of the method was 0.5 ng/L.
Keywords :
optimisation; response surface methodology; sewage treatment; statistical analysis; internal standard method; mathematical method; multiple reaction monitoring; oestrone quantification; response surface method; river water matrice; sewage; solid phase extraction; statistical method; steroid estrogen; Design for experiments; Design optimization; Methanol; Optimization methods; Reservoirs; Response surface methodology; Rivers; Statistical analysis; Testing; Voltage;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5515175
