DocumentCode :
309467
Title :
Detection of nonspecific immunohistochemical response caused by tissue necrosis and the presence of metallic wear debris
Author :
Tucci, Michelle ; Cason, Zelma ; Tsao, Audrey ; Benghuzzi, Hamed
Author_Institution :
Dept. of Orthopaedic Surg. & Health Sci., Mississippi Med. Center, Jackson, MS, USA
fYear :
1997
fDate :
4-6 Apr 1997
Firstpage :
187
Lastpage :
189
Abstract :
Tissues retrieved during revision arthroplasty (n=25) were processed for routine immunohistochemical techniques to localize the presence of bone resorbing cytokines (IL-1 and IL-6), and to identify areas of significant necrosis and metallic deposition. Tissues were processed and embedded according to standard laboratory techniques. Several slides were prepared for each tissue sample taken. Several sequential slides were stained for Hematoxylin and Eosin to demonstrate cell type. Other slides of the same tissues were processed for localization of cytokines, IL-1 and IL-6. To identify specific cytokine reactions, the tissues were first blocked with proteins to ensure that all. The results revealed that there were several locations on the tissues that appeared highly reactive for cytokines IL-1 and IL-6. However, some of these areas turned out to be nonspecific when compared with negative controls and the Hematoxylin and Eosin stained slides. Areas of extreme necrosis or where metal debris was presence demonstrated sites of nonspecific reactivity. Areas of necrosis have a high nonspecific affinity for antibodies and these areas of necrosis can be demonstrated on the Hematoxylin and Eosin sections, and should be avoided in determining the amount of specific cellular reactivity. In addition, new immunohistochemical techniques use cobalt ions as enhancers of peroxidase response, in areas on slides labeled as negative controls where metallic debris was trapped, there was an increase in nonspecific positive reactivity. These factors must be carefully controlled in determining true cellular responses that causes resorption of bone and implant failure
Keywords :
bone; laboratory techniques; prosthetics; wear; Eosin; Hematoxylin; antibodies; bone resorption; cell type; cytokines; implant failure; metallic wear debris; nonspecific immunohistochemical response detection; nonspecific positive reactivity; retrieved tissues; revision arthroplasty; slides; surgical specimens; tissue necrosis; Bones; Cobalt; Extracellular; Immune system; Implants; Laboratories; Orthopedic surgery; Polyethylene; Proteins; Recruitment;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Biomedical Engineering Conference, 1997., Proceedings of the 1997 Sixteenth Southern
Conference_Location :
Biloxi, MS
ISSN :
1086-4105
Print_ISBN :
0-7803-3869-3
Type :
conf
DOI :
10.1109/SBEC.1997.583247
Filename :
583247
Link To Document :
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