Title :
Integrating biological molecules with electrode surfaces for bioanalytical sensing applications
Author :
Robertson, Joseph W F ; Silin, Vitalii ; Reiner, Joseph E. ; Kasianowicz, John J.
Author_Institution :
Phys. Meas. Lab., Nat. Inst. of Stand. & Technol., Boulder, CO, USA
Abstract :
A detection scheme for Anthrax toxins based on the interaction of an ion channel formed by protective antigen 63 (PA63)7, a heptameric ion channel forming toxin, with lethal factor is demonstrated.To prepare a sensor, a biomimetic membrane is formed on an electrode surface through a thiolipid molecule with a custom synthesized poly(ethylene oxide) spacer. Thus a membrane is formed across the electrode with an aqueous space of ~ 2 nm. The high-specificity of the TF-(PA6 3)7 interaction enables the detection of Anthrax proteins on the order of pmol/L. In addition to highly sensitive detectors, this tethered membrane construct is scaleable and can be extended to devices with micron and sub-micron sensing pads, critical for the miniaturization required for novel bioelectronic devices.
Keywords :
bioelectric phenomena; biomembrane transport; biomimetics; biosensors; electrochemical electrodes; molecular biophysics; proteins; (PA63)7; Anthrax proteins; Anthrax toxins; bioanalytical sensing; bioelectronic devices; biological molecules; biomimetic membrane; electrode surfaces; high-specificity; ion channel; lethal factor; poly(ethylene oxide) spacer; protective antigen 63; thiolipid molecule; Biomembranes; Electrodes; Optical sensors; Proteins; Semiconductor device measurement; USA Councils;
Conference_Titel :
Semiconductor Device Research Symposium (ISDRS), 2011 International
Conference_Location :
College Park, MD
Print_ISBN :
978-1-4577-1755-0
DOI :
10.1109/ISDRS.2011.6135209
