Title :
The Research on Proteomic Changes in Two Bioflocculant Producing Bacteria When Grown in Minimal Media Versus Bioflocculant Media
Author :
Yu, Bing ; Ma, Fang ; Qi, Peishi ; Li, Ang ; Wang, Lili
Author_Institution :
Sch. of Municipal & Environ. Eng., Harbin Inst. of Technol., Harbin, China
Abstract :
In order to reveal the molecular mechanisms and the metabolic pathway of producing bioflocculant, the changes in protein complexes of F2 grown in minimal media versus bioflocculant media and those of F6 grown in minimal media versus bioflocculant media were respectively researched by SDS-PAGE. SDS-PAGE results indicated that the relative band volumes of three bands were obviously decreased in F2, the relative band volume of one band was obviously increased in F2 as well as the relative band volumes of two bands were obviously decreased in F6, the relative band volume of one band was obviously increased in F6. The conclusion was that seven protein complexes, which are differentially expressed and important to induce and produce the bioflocculant, were obtained and need further indentify the protein components in them.
Keywords :
biochemistry; flocculation; microorganisms; proteins; proteomics; SDS-PAGE; bacteria; bioflocculant media; metabolic pathway; minimal media; molecular mechanisms; protein complexes; proteomics; relative band volume; Biological materials; Capacitive sensors; Electrokinetics; Infrared spectra; Laboratories; Microorganisms; Proteins; Proteomics; Water pollution; Water resources;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5515538