Isolation, Identification and Degradation Characterization of 1,2-Dichlorobenzene Degrading Strain

A bacterium(strain DL-1) capable of growing with 1,2-dichlorobenzene as sole carbon and energy sources was isolated from rhizosphere soil in phragmites wetland by using the enrichment culture contaminated with 1,2-dichlorobenzene concentration gradient of 20, 40, 60, 80, 120, 160 and 200 mg·L^-1. It was identified as Bacillus cereus by 16S rDNA gene sequence analysis and the bacterial culture characteristics, carried physiological and biochemical research, the strain was Gram-positive, peritrichous flagellum. The further degrading characterization research proved the strain was a facultative anaerobe and the optimal temperature, pH value and inoculation amount of 1,2-dichlorobenzene degradation were 30°C, pH7.0 and 10%, in 4 d. Respectively , the optimal concentration of 1,2-dichlorobenzene in four days was 120 mg·L^-1.