Cross-Talk Calibrating Based on Correlation Analysis in High-Throughput Genome Sequencing

Author

Ye, Bing-gang ; Wu, Xiao-ming ; Li Jing-Xiang ; Zhou Yan ; Wang De-peng

Author_Institution

Sch. of Biosci. & Bioeng., South China Univ. of Technol., Guangzhou, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

4

Abstract

The images, in high-throughput genome sequencing of sequencing by synthesize, consist of A, C, G, T base fluorescence images, and the fluorophore emission spectra partially overlap, which causes cross-talk problem. This paper presents a method of correlation analysis, construct the calibration matrix to eliminate interference of the fluorophore. The experiment result shows the method provided in this article is effective to correct cross-talk influence, and get the deconvolution of the clusters signal.

Keywords

DNA; bio-optics; bioinformatics; crosstalk; deconvolution; fluorescence; genetics; genomics; molecular biophysics; DNA; calibration matrix; clusters signal deconvolution; correlation analysis; cross-talk calibration; fluorescence; fluorophore emission spectra; high-throughput genome sequencing; Bioinformatics; Biomedical engineering; Calibration; Deconvolution; Fluorescence; Genomics; Image analysis; Image sequence analysis; Interference elimination; Signal synthesis;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5515864

Filename

5515864