Title :
Effects of Water-Borne Copper on Isozymes of Gill in Eriocheir sinensis
Author :
Zhao, Yun-long ; Yang, Zhi-Biao ; Yang, Jian ; Hua, Xiu-Guo ; Yuan, Cong-Li ; Zhang, Wen ; Li, Na ; Fan, Yu-jie ; Zhu, Jian-guo ; Cui, Li
Author_Institution :
Sch. of Life Sci., East China Normal Univ., Shanghai, China
Abstract :
By using the PAGE (Poly Acrylamide Gel Electrophoresis), the effect of water-borne Cu2+ on the isozymes in gills of E. sinensis was studied. The experimental groups were treated with different concentration of Cu2+ as 0.01, 0.10, 1.00, and 5.00 mg/l, respectively. The results showed that different isozymes had specific responses. The activity of LDH and MDH decreased gradually along with the increasing of Cu2+ level. Increasing of Cu2+ level followed by decreasing of ALP was observed, and the variation of EST wasn´t significant. These isozymes variations reflected the toxic effects of Cu2+ sensitively.
Keywords :
aquaculture; biochemistry; copper; electrophoresis; enzymes; gels; molecular biophysics; physiology; polymers; toxicology; water pollution; zoology; ALP; Cu; E. sinensis; EST; Eriocheir sinensis; LDH; MDH; PAGE; gill; isozymes; polyacrylamide gel electrophoresis; water-borne Cu2+; water-borne copper; Agriculture; Aquaculture; Biochemistry; Biotechnology; Copper; Electrokinetics; Laboratories; Plasma measurements; Water pollution; Water resources;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5515879
