The Effect of Mono-(2-Ethylhexyl) Phthalate on Human Granulosa Cells

Various phthalates are used extensively as plasticizers in kinds of consumer products. They are harmful for human reproduction and fertility. Mono-(2-ethylhexyl) phthalate (MEHP) is the active metabolite of di-(2-ethylhexyl) phthalate(DEHP),which is most commonly used as a plasticizer and solvent in numerous consumer products. MEHP has been demonstrated to be a reproductive toxicant in rodents, but the evidences of using human cells are rare. In present study, we examined the effect of MEHP on viability and steroid production of human granulosa cells. Human granulosa cells obtained from nine patients undergoing in vitro fertilization were cultured in medium with various concentrations of MEHP (100,300,500 μmol L^-1). After culture for 48 hours, granulosa cells viability was determined. Estradiol, progesterone and testosterone were assayed in the spent culture medium. We found that the viability of granulosa cells was reduced obviously at 100, 300 and 500μmol L^-1 of MEHP. High concentration of MEHP(500uM) was harmfull to steriod hormone synthesis, such as estradiol and progestorone. These findings will provide favorable evidence for adverse effect of MEHP on women´s reproductive system.