A CAD study on micro flow cytometer and its application to bacteria detection

Author

Lee, Sang-Seok ; Izuo, Shin-Ichi ; Inatomi, Ken-Ichi

Author_Institution

Adv. Technol. R&D Center, Mitsubishi Electr. Corp., Hyogo, Japan

fYear

2004

fDate

24-27 Oct. 2004

Firstpage

308

Abstract

We have designed and fabricated a micro flow cytometer for application to bacteria detection. In this paper, we present a CAD study which has been done to obtain optimal working conditions of the device. In the simulation, we have investigated flow rate ratios of sample and sheath flow to confirm hydrodynamic focusing at a desired position and proper sample stream width at the detection position. As a result, we could obtain adequate flow rate ratios of sample and sheath flow, which is in the vicinity of 1:5 for our device. A bacteria enumeration experiment has also been carried out, based on the simulation results, and we have achieved hydrodynamic focusing at the expected position and proper sample flow rate.

Keywords

CAD; biosensors; cellular biophysics; computational fluid dynamics; hydrodynamics; microfluidics; microorganisms; microsensors; CAD; bacteria detection; bacteria enumeration; bacterial cell counting; detection position sample stream width; hydrodynamic focusing; microfluidic channel; micromachined micro flow cytometer; sample/sheath flow rate ratio; Biological cells; Design automation; Employee welfare; Fabrication; Fluorescence; Hydrodynamics; Microfluidics; Micromachining; Microorganisms; Microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors, 2004. Proceedings of IEEE

Print_ISBN

0-7803-8692-2

Type

conf

DOI

10.1109/ICSENS.2004.1426163

Filename

1426163