Curcumin Upregulate HO-1 Expression but Downregulate HO-2 Expression in SHSY5Y Cell

Author

Shi, Xiaodong ; Li, Yu

Author_Institution

Dept. of Pathology, Chongqing Med. Univ., Chongqing, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

4

Abstract

Curcumin, the most active constituent of turmeric, is currently one of the major anticance new drugs and has been reported to enhance Heme Oxygenase-1(HO-1) in human brain when oxidative stress occur. Keeping the balance of HO-1 and HO-2 plays an important role in the brain. However, few researches study the effects of curcumin on the two isoenzymes of HO. Here we investigated the effects of curcumin on the change of HO-1 and HO-2 in neuroblastoma cell line SHSY5Y. We found that the mRNA and protein levels of HO-1 were increased in a dose-and time-dependent manner (P<;0.05) in the cells after treated with Curcumin, whereas, the expression of HO-2 were gradually downregulated in a dose-and time-dependent manner (P<;0.05). A negative correlation was found between HO-1 and HO-2. These results indicate that Curcumin regulated HO-1 and HO-2 bilaterally, which may have an important effect on the pathogenesis and development of carcinoma, and may provide a new direction for disposing of carcinoma.

Keywords

brain; cellular biophysics; drugs; enzymes; macromolecules; molecular biophysics; proteins; HO-1 expression; HO-2 expression; SHSY5Y cell; carcinoma; curcumin; heme oxygenase-1; human brain; isoenzymes; mRNA; neuroblastoma cell line; oxidative stress; protein levels; Biomembranes; Cancer; Degradation; Humans; Medical treatment; Neuroscience; Organisms; Pathology; Proteins; Stress;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5516396

Filename

5516396