Electroporation-Induced Cell Lysis in SWLA-2 Hybridomas

Author

Oakley, Barbara A. ; Gross, Brooks A. ; Martens, Sara G. ; Hanna, Darrin M. ; Stryker, Gabrielle A.

Author_Institution

Sch. of Eng. & Comput. Sci., Oakland Univ., Rochester, MI

fYear

2006

fDate

Aug. 30 2006-Sept. 3 2006

Firstpage

3495

Lastpage

3496

Abstract

This paper describes experimental results involving the percentage cell lysis in SWLA-2 murine hybridomas produced by square wave electric field pulses of 100, 200, and 300 V across a 1 mm gap width in a standard cuvette. Pulse lengths were of 0.2 and 0.6 ms duration; 1, 2, or 3 pulses were applied with 100 ms time interval between pulses. Cells were cultured and separate samples examined at 48 hours to determine cell mortality. Nearly 90% cell mortality was produced by applying 3 pulses at of 0.6 ms duration at 300 V

Keywords

bioelectric phenomena; biological effects of fields; cellular effects of radiation; 0.2 ms; 0.6 ms; 1 mm; 100 V; 200 V; 300 V; 48 hr; SWLA-2 murine hybridomas; cell culture; cell mortality; electroporation-induced cell lysis; square wave electric field pulses; Amino acids; Biology; Cells (biology); Cities and towns; Computer science; Electrochemical machining; Electrodes; Space vector pulse width modulation; USA Councils; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2006. EMBS '06. 28th Annual International Conference of the IEEE

Conference_Location

New York, NY

ISSN

1557-170X

Print_ISBN

1-4244-0032-5

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2006.259722

Filename

4462549