Confocal nonlinear optical microscopy for high resolution measurement of tea-leaf cells

Author

Masaki, Minoru ; Egami, Chikara

Author_Institution

Fac. of Eng., Shizuoka Univ., Hamamatsu

fYear

2006

fDate

Oct. 2006

Firstpage

237

Lastpage

238

Abstract

High-resolution optical microscopy is very important in various applications including biological-cell imaging. In this paper we propose a new type of confocal microscopic spectroscopy to use a nonlinear absorption of the biological cells without doping fluorescent dyes. The confocal nonlinear optical microscopy by use of wave mixing geometry can observe a nanostructure of the cell inside. A three dimensional structure of a green-tea-leaf cell which had a weak absorption at a wavelength of 633 nm is measured

Keywords

biological techniques; botany; cellular biophysics; dyes; fluorescence; image resolution; multiwave mixing; optical microscopy; 633 nm; biological-cell imaging; confocal microscopic spectroscopy; confocal nonlinear optical microscopy; fluorescent dyes; green tea-leaf cell; high resolution measurement; nanostructured cell; nonlinear absorption; wave mixing geometry; Absorption; Biological cells; Biomedical optical imaging; Doping; High-resolution imaging; Nonlinear optics; Optical imaging; Optical microscopy; Optical mixing; Spectroscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics Society, 2006. LEOS 2006. 19th Annual Meeting of the IEEE

Conference_Location

Montreal, Que.

Print_ISBN

0-7803-9555-7

Electronic_ISBN

0-7803-9555-7

Type

conf

DOI

10.1109/LEOS.2006.279016

Filename

4054144