Title :
Confocal nonlinear optical microscopy for high resolution measurement of tea-leaf cells
Author :
Masaki, Minoru ; Egami, Chikara
Author_Institution :
Fac. of Eng., Shizuoka Univ., Hamamatsu
Abstract :
High-resolution optical microscopy is very important in various applications including biological-cell imaging. In this paper we propose a new type of confocal microscopic spectroscopy to use a nonlinear absorption of the biological cells without doping fluorescent dyes. The confocal nonlinear optical microscopy by use of wave mixing geometry can observe a nanostructure of the cell inside. A three dimensional structure of a green-tea-leaf cell which had a weak absorption at a wavelength of 633 nm is measured
Keywords :
biological techniques; botany; cellular biophysics; dyes; fluorescence; image resolution; multiwave mixing; optical microscopy; 633 nm; biological-cell imaging; confocal microscopic spectroscopy; confocal nonlinear optical microscopy; fluorescent dyes; green tea-leaf cell; high resolution measurement; nanostructured cell; nonlinear absorption; wave mixing geometry; Absorption; Biological cells; Biomedical optical imaging; Doping; High-resolution imaging; Nonlinear optics; Optical imaging; Optical microscopy; Optical mixing; Spectroscopy;
Conference_Titel :
Lasers and Electro-Optics Society, 2006. LEOS 2006. 19th Annual Meeting of the IEEE
Conference_Location :
Montreal, Que.
Print_ISBN :
0-7803-9555-7
Electronic_ISBN :
0-7803-9555-7
DOI :
10.1109/LEOS.2006.279016
