Title :
Cloning and Sequence Analysis of a cDNA of P450 17a-Hydroxylase/17,20-Lyase(CYP17) from Strongylocentrotus nudus
Author :
Guo, Xiaoli ; Liu, Yang ; Qiu, Xuemei ; Wang, Xiuli
Author_Institution :
Coll. of Life Sci. & Biotechnol., Dalian Fisheries Univ., Dalian, China
Abstract :
CYP17 (17α-hydroxylase/17, 20-lyase) has been viewed as a critical enzyme for the biosynthesis of gonadal and adrenal steroids in vertebrates. According to a reported CYP17 gene of Strongylocentrotus purpuratus gene from NCBI, three pairs of primers were designed to clone CYP17 gene of Strongylocentrotus nudus,using RT-PCR. Three fragments were assembled into on contig of 1569bp, including full CDS of 1482bp encoding 493 amino acid residues. Sequence analysis indicated the two sea urchin CYP17 genes were mostly similar(97%) . But their amino acid identities were low compared with vertebrates. Cloning of this CYP17 gene would be necessary to characterize its function in development of gonads of sea urchin.
Keywords :
DNA; biochemistry; cellular biophysics; enzymes; genetics; genomics; proteomics; CYP17 gene; P450 17a-hydroxylase-17,200-lyase; RT-PCR; Strongylocentrotus Nudus; adrenal steroids; amino acid residues; biosynthesis; cDNA; cloning; enzyme; gonadal steroids; sea urchin; sequence analysis; vertebrates; Amino acids; Aquaculture; Assembly; Biochemistry; Cloning; Encoding; Peptides; Production; RNA; Sequences;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5516721
