The optimal axial interval in estimating depth from defocus

We analyze the effect of perturbations on the estimation of Depth from Defocus (DFD) implemented by changing the focus setting (e.g., axially moving the sensor). The analysis yields the optimal change of focus setting, and the spatial frequencies for which estimation is most robust. For stable estimation at all spatial frequencies, the change in focus setting should be less than twice the depth of field. For the most robust estimation in the highest spatial frequencies the axial interval should be equal to the depth of field