Title :
Isolation, Identification and Characterization of an Efficient Phenanthrene-Degrading Bacterial Strain
Author :
Zhang, Wei ; Lin, Kuang-fei ; Long, Yi-Tao ; Li, Xiong ; Liu, Li-li
Author_Institution :
State Environ. Protection Key Lab. of Environ. Risk Assessment & Control on Chem. Process, East China Univ. of Sci. & Technol., Shanghai, China
Abstract :
The degradation dynamics of phenanthrene in soil was studied. The half-life of phenanthrene in the non-sterilized contaminated soil was only 5.1 d, much shorter than the same in the sterilized soil. The dominant strain of phenanthrene microbial degradation was isolated and identified by 16S rDNA as Alcaligenes sp.; its growth and degradative capability were highest at 25°C and pH of 7.0. Inoculation of the dominant strain to soil enhanced phenanthrene degradation, and faster degradation rates were observed with larger doses of the strain inoculated.
Keywords :
DNA; biological techniques; cellular biophysics; microorganisms; molecular biophysics; pollution control; soil pollution; 16S rDNA; Alcaligenes sp; bacterial strain; microbial degradation; phenanthrene; polycyclic aromatic hydrocarbons; soil; temperature 25 degC; Biodegradation; Capacitive sensors; Chemical technology; Detectors; Microorganisms; Pollution; Protection; Soil; Temperature; Thermal degradation;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5516749