Influence of Surfactant-Tween80 on Degradation of Benzo(a)Pyrene and Its Accumulated Metabolites by Bacillus-07

Two degradation metabolites of benzo(a)pyrene(BaP), cis-BP4,5-dihydrodiol and cis-BP7,8-dihydrodiol, were identified using high performance liquid chromatography (HPLC) with standards. Both BaP and the two metabolites were well-known toxic compounds. The effect of a nonionic surfactant-Tween80 (TW80) on the degradation of the above three compounds by Bacillus-07, in aqueous phase, was studied. The result showed that (1) Solubility of the three compounds was enhanced by more than 20 times and their bioavailability was markedly improved; (2) Compared with controls, degradation rate of BaP was enhanced by 25.01%, meanwhile accumulation rates of cis-BP4,5-dihydrodiol and cis-BP7,8-dihydrodiol were lowered 2.11% and 2.57% on the 8d, respectively, after the adding of 1000 ppm TW80; (3) TW80 could be utilized by microorganisms as energy and it exhibited no inhibitory effect on the growth of Bacillus-07.