Title :
Comparative Study on Expression of Relevant Proteins of Proliferation and Differentiation of Human Epidermal Stem Cells between Diabetic Skin and Normal Skin
Author :
Zhong, Qingling ; Liu, Fanrong ; Liu, Dewu ; Peng, Yan ; Zhu, Feibin
Author_Institution :
Burns Inst., Nanchang Univ. Med. Coll., Nanchang, China
Abstract :
β-catenin plays an important role in the tissue repair. But its expression and meanings in the diabetic cutaneous wound remains to be determined. In this study, we investigated the expression of β-catenin and PCNA in epidermal stem cells (ESCs) of diabetic rat skin. The diabetes mellitus (DM) rats were induced by a single i.p. injection of streptozotocin. ESCs were extracted from the dorsal skin samples of rats by trypsin digesting method and further purified by collagen adhering methods. Expression of β1-integrin and keratin 19 were identified as ESCs by immunocytochemical staining . The results showed the amount of β-catenin and PCNA were decreased in ESCs of DM rat skin, and the clone forming efficiency of ESCs in DM rat skin was significantly lower than that in normal skin. It reveals impaired wounds repair in DM is characterized by the protracted epithelialization which is due to a decreased proliferative capacity of epidermal stem cells.
Keywords :
biological tissues; cellular biophysics; diseases; proteins; skin; wounds; β-catenin; β1-integrin; clone forming efficiency; collagen adhering; cutaneous wound; diabetes mellitus; diabetic skin; differentiation; human epidermal stem cells; immunocytochemical staining; keratin 19; proliferation; proteins; streptozotocin; trypsin digesting; Delta modulation; Diabetes; Epidermis; Humans; Immune system; Proteins; Rats; Skin; Stem cells; Wounds;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5517110
