The Purification of Alkaline and Thermostable Lipase from a Newly Isolated Strain Acinetobacter calcoaceticus 1-7

Author

Wang, HaiKuan ; Ma, HuiJing ; Zhong, ShaoJiong ; Wei, YuJie ; Qi, Wei

Author_Institution

Key Lab. of Ind. Microbiol., Tianjin Univ. of Sci. & Technol., Tianjin, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

3

Abstract

A newly isolated moderate thermophilic strain 1-7 producing alkaline and thermostable lipase previously was identified as Acinetobacter calcoaceticus, which was isolated from the soil collected from bay of Bohai in China. The lipase showed maximum activity at pH 9.0 and high thermostability. The lipase from Acinetobacter calcoaceticus 1-7 was purified by ammonium sulfate precipitation, ion exchange chromatography and Sephadex G-75 chromatography. The molecular mass (Mr) of the lipase was estimated to be 30 kDa using SDS-PAGE.

Keywords

biochemistry; biological techniques; biotechnology; chromatography; enzymes; microorganisms; precipitation (physical chemistry); proteomics; purification; thermal stability; Acinetobacter calcoaceticus 1-7; Bohai; China; Sephadex G-75 chromatography; alkaline lipase; ammonium sulfate precipitation; ion exchange chromatography; isolated moderate thermophilic strain 1-7; molecular mass; purification; thermostable lipase; Biochemistry; Capacitive sensors; Chemical industry; Conducting materials; Microorganisms; Petroleum; Purification; Soil; Temperature; Water;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5517247

Filename

5517247