Research on the urease biosensor with PPy material as carrier and sensitive membrane

Aimed at the dual biofunctional membrane comprised with carrier layer and sensing layer used in BioFET(Bio Field Effect Transistor) nowadays, this paper designed a kind of novel one-layer membrane comprising polypyrrole and urease with electrochemical method, which can replace traditional double-layer membrane, and give a new method for the design of the functional membrane of BioFET. The BioFET sensor show a linearity of 0.9995 in the urea concentration range of 10^-7 mol/L~10^-3mol/L, and a high sensitivity of 45.9mV/pUrea was obtained.