Title :
Determination of Trace Oxytetracycline in Wasterwater by Phase-Separation Floatation with Ultraviolet Detection
Author :
Hou Yan-min ; Cheng Yong-hua ; Yan Yong-sheng
Author_Institution :
Sch. of Chem. & Chem. Eng., Pingdingshan Univ., Pingdingshan, China
Abstract :
In the present work, a novel analytical method was proposed for the determination of trace oxytetracycline(OTC) in wastewater by phase-separation floatation(PSF) coupled with an ultraviolet detector setting at 375 nm. OTC can be transformed into tenary complex(Co-OTC-DNA) in the presence of cobalt(II) and deoxyribonucleic acid (DNA) which can be separated by phase-separation floatation easily. The optimum phase-separation floatation was performed on phase-separation reagent using 1 mol·L-1 sodium chloride, caustic soda solution as acidity regulator and tetrahydrofuran(THF) as solvent. The linear regression equation is A=7.85×105C-0.002, linear range is from 5.0×10-8-1.0×10-5 mol·L-1, lower limit of detection (LOD) was 3.82×10-9 mol·L-1. The recoveries of OTC in water samples were 99.1-99.4%.
Keywords :
DNA; phase separation; photodetectors; regression analysis; ultraviolet detectors; wastewater; water pollution measurement; acidity regulator; caustic soda solution; deoxyribonucleic acid; linear regression equation; phase-separation floatation; tenary complex; tetrahydrofuran; trace oxytetracycline; ultraviolet detector; wastewater; wavelength 375 nm; Absorption; Chemical analysis; Chemical engineering; Chemistry; DNA; Detectors; Nitrogen; Phase detection; Solvents; Wastewater;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5517299