Screening and 16S rRNA Analysis of the Bacteria of Producing Chitin Deacetylase

Chitin deacetylase (CDA) is an enzyme that catalyzes the hydrolysis of acetamine groups of N-acetyl-D-glucosamine in chitin. Chitin deacetylase converting chitin to chitosan in fungal cell walls. A total of 208 strains was isolated from soil samples in the riverbank of Xiangjiang River and an island of Zhanjiang. 12 CDA producing strains was founded by color reaction that including three fungus, two bacterias and four actinomycetes. The highest CDA producing strain Z7 had been screened by enzyme activity assays. 16S gene of the strain was amplified and sequenced. The 16S gene sequence data were deposited in the GenBank nucleotide sequence database. The 16S gene sequences were aligned with the multiple - sequence alignment software Clustal S. Similarity values were calculated using the software MegAlign. Phylogenetic and molecular evolutionary analyses were conducted using MEGA by the neighbor - joining algorithms. Aphylogenetic tree was constructed by comparing with the published 16S gene regions of the related bacteria species. In the phylogenetic tree the overall similarity value between strain Z7 and Bacillus amyloliquefaciens are 99%. The wild strain was classfy as Bacillus amyloliquefaciens.