Title :
Effect of Water-Accommodated Fractions of No.0 Diesel Oil on HOX1 Gene of Hemicentrotus pulcherrimus Sea Urchin
Author :
Gang, M. ; Xiong, D.Q. ; Lv, F.R.
Author_Institution :
Coll. of Environ. Sci. & Eng., Dalian Maritime Univ., Dalian, China
Abstract :
The paper aims at presenting the acute toxic effect of water-accommodated fractions (WAFS) of No.0 diesel oil on HOX1 gene of Hemicentrotus pulcherrimus by PCR-SSCP. The experiment was carried out with three concentrations(2mg/L, 5mg/L, 10mg/l). The results indicated that with the increase of exposure concentration and time, the probability of mutation increased. In the four primers, single base mutation took place in coding region of the 184 in primer N-2, with the conversion of T-C, where amino acid changed from serine into proline.
Keywords :
genetics; marine pollution; oil pollution; petroleum; toxicology; HOX1 gene; Hemicentrotus pulcherrimus; PCR-SSCP; diesel oil; mutation probability; sea urchin; single base mutation; toxic effect; water-accommodated fractions; Amino acids; Conducting materials; DNA; Embryo; Genetic mutations; Hazardous materials; Materials testing; Ocean temperature; Petroleum; Sequences;
Conference_Titel :
Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-4712-1
Electronic_ISBN :
2151-7614
DOI :
10.1109/ICBBE.2010.5517379
