Effect of Water-Accommodated Fractions of No.0 Diesel Oil on HOX1 Gene of Hemicentrotus pulcherrimus Sea Urchin

Author

Gang, M. ; Xiong, D.Q. ; Lv, F.R.

Author_Institution

Coll. of Environ. Sci. & Eng., Dalian Maritime Univ., Dalian, China

fYear

2010

fDate

18-20 June 2010

Firstpage

1

Lastpage

3

Abstract

The paper aims at presenting the acute toxic effect of water-accommodated fractions (WAFS) of No.0 diesel oil on HOX1 gene of Hemicentrotus pulcherrimus by PCR-SSCP. The experiment was carried out with three concentrations(2mg/L, 5mg/L, 10mg/l). The results indicated that with the increase of exposure concentration and time, the probability of mutation increased. In the four primers, single base mutation took place in coding region of the 184 in primer N-2, with the conversion of T-C, where amino acid changed from serine into proline.

Keywords

genetics; marine pollution; oil pollution; petroleum; toxicology; HOX1 gene; Hemicentrotus pulcherrimus; PCR-SSCP; diesel oil; mutation probability; sea urchin; single base mutation; toxic effect; water-accommodated fractions; Amino acids; Conducting materials; DNA; Embryo; Genetic mutations; Hazardous materials; Materials testing; Ocean temperature; Petroleum; Sequences;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering (iCBBE), 2010 4th International Conference on

Conference_Location

Chengdu

ISSN

2151-7614

Print_ISBN

978-1-4244-4712-1

Electronic_ISBN

2151-7614

Type

conf

DOI

10.1109/ICBBE.2010.5517379

Filename

5517379