A novel detector for Chromatography and estradiol immune sensor based on surface plasma resonance

Biosensors based on SPR (surface plasma resonance) are attracting more and more attention from academic and industry communities. Its principle was interpreted. A novel detector based on SPR was presented for Chromatography. Associated with ion chromatography system, it was employed to detect separation behavior of mixture including glucose, fructose and galacitiol. Experimental results demonstrated that RSD (relative standard deviation) of each separated glucide was below 2.5%. The detection limits of glucose, galacitiol and fructose were 6μg, 10μg and 15μg respectively. The linear correlation coefficient of each separated glucide was greater than 0.995 in the range from detection limit to upper concentration. Combined with the immune detection technology, the SPR flow injection analysis system was designed to determine standard curve of environmental pollutant estradiol. Detection limit of estradiol successfully reached 0.625ng.