Effect of hesperidin extraction on cell proliferation and apoptosis of CNE-2Z cells

Objective: To study the effect of hesperidin extracted from the peel of Gannan kumquat on cell proliferation and apoptosis of human poorly diferentiated nasopharyongeal carcinoma epithelial cell (CNE-2Z). Methods: Effect of hesperidin on proliferation of CNE-2Z cells was detected by the MTT method. Effect of hesperidin on apoptosis of CNE-2Z cells was utilized by the acridine orange / ethidium bromide staining method. Results: After being treated with different hesperidin for 24, 48 and 72 h respectively, the proliferation of CNE-2Z cells was inhibited in vitro; different time had high significant difference on the proliferation of CNE-2Z cells (F = 1898.865, P <;; 0.001), different pharmacal concentration had had high significant difference on the proliferation of CNE-2Z cells (F = 5454.040, P <;; 0.001). Time and pharmacal concentration had interaction on the proliferation of CNE-2Z cells (F = 118.909, P <;; 0.001). During the concentration of 1μmol/L-100μmol/L, inhibition ratio of CNE-2Z cells increased gradually along with the increment of pharmacal concentration and the extension of time. When the concentration was higher than 100 μmol/L, its inhibition no more reinforced accompanied with the increasement of pharmacal concentration (P> 0.05) and presented the determinate saturability. The aggregation of cellular nuclear chromatin, karyopycnosis, karyorrhexis and apoptotic body was present in CNE-2Z cells detected by acridine orange/ethidium bromide fluorescent staining after the utilization of hesperidin. Conclusion : Hesperidin inhibited the cellular division growth and induced the cell apoptosis of CNE-2Z cells.