Title :
Induction of apoptosis in human gastric carcinoma cell by Geraniin
Author :
Bao Qing Wang ; Zhe Xiong Jin
Author_Institution :
Coll. of Pharmacy, Harbin Univ. of Commerce, Harbin, China
Abstract :
Aim: To study the anti-tumour effect of Geraniin isolated from Agrimonia pilosa ledeb. Methods: MTT was used to study anti-tumor effect of Geraniin in vitro. Morphological observation of the cells was completed by fluorescence microscope. The effect of Gerannin on cell apoptosis in human gastric carcinoma SGC-7901 cells was studied by flow cytometry. Results: The proliferation of SGC-7901 cells was inhibited after treated with 0.01, 0.1, 1, 10 and 15μg/ml Gerannin for 72h and the IC50 is 11.5μg/mL. After SGC-7901 cells were treated by geraniin for 24h, morphological changes of apoptosis were observed. The apoptotic cells were detected after treating with 0.01, 0.1, 1 and 10μg/ml of Gerannin for 24h. The rate of apoptosis in SGC-7901 cells induced by gerannin was found to be 7, 11.1, 20, and 27.1%, respectively. Conclusion: Gerannin has anti-tumour effect. Its mechanism of anti-tumour could be attributed to its action of initiating the apoptosis of tumor cells.
Keywords :
biochemistry; biomedical optical imaging; cancer; cellular biophysics; patient treatment; tumours; Agrimonia pilosa ledeb; Geraniin; SGC-7901 cells; antitumor effect; cell apoptosis; flow cytometry; fluorescence microscope; human gastric carcinoma cell; Business; Cancer; Chemicals; Educational institutions; Humans; Morphology; Agrimonia pilosa ledeb; Anti-tumour; Geraniin; cell apoptosis;
Conference_Titel :
Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on
Conference_Location :
Yantai
Print_ISBN :
978-1-4244-6495-1
DOI :
10.1109/BMEI.2010.5639480
