Title :
DNA quantification with an electrochemiluminescence microcell
Author :
Hsueh, Yun-Tai ; Collins, Scott D. ; Smith, Rosemary L.
Author_Institution :
Dept. of Electr. Eng. & Comput. Sci., California Univ., Davis, CA, USA
Abstract :
Describes the detection and quantification of DNA strands using an electrochemiluminescence (ECL) instrument with microfabricated excitation/detection cell. The microcell contains thin film Pt electrodes, fluid microchannels and a silicon PIN diode photodetector. The DNA strands are labeled with an electrochemically exited, luminescing molecule: tris( 2, 2´ bipyridyl) ruthenium (TBR). In order to separate the free label from labeled DNA, the DNA strands are attached to paramagnetic beads with a biotin-streptavidin linkage. With a 1 cm2 Pt anode, a detection limit of 40 femtomoles of DNA is obtained with a silicon PIN diode photodetector operating at room temperature. The overall size of the microcell is only 1.5×2×0.3 cm with a sample volume of 150 μL
Keywords :
DNA; biological techniques; chemiluminescence; electrochemistry; microelectrodes; molecular biophysics; p-i-n photodiodes; photodetectors; 0.3 cm; 1 cm; 1.5 cm; 2 cm; DNA quantification; DNA strands; Pt; Si; Si PIN diode photodetector; biotin-streptavidin linkage; detection; detection limit; electrochemically exited luminescing molecule; electrochemiluminescence instrument; electrochemiluminescence microcell; fluid microchannels; free label; labeled DNA; microcell size; microfabricated excitation/detection cell; paramagnetic beads; room temperature; sample volume; thin film Pt electrodes; tris( 2, 2´ bipyridyl) ruthenium; Couplings; DNA; Electrodes; Instruments; Microcell networks; Microchannel; Paramagnetic materials; Photodetectors; Semiconductor thin films; Silicon;
Conference_Titel :
Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on
Conference_Location :
Chicago, IL
Print_ISBN :
0-7803-3829-4
DOI :
10.1109/SENSOR.1997.613611
