Title :
Effect of ethanol extract concentrations of Hei Chou on calcification of MC3T3-E1 cell
Author :
Ahn, Kwang-Sik ; Lee, Young-Hoon ; Kwon, Ha-Jeong ; Park, Yong-duk
Author_Institution :
Dept. of Preventive Dentistry, Kyung Hee Univ., Seoul, South Korea
Abstract :
Some crude drugs have been known to useful effect of bone tissue regeneration in oriental medicine. These drugs were studied many scientific approach for bone formation. In this study, Alizarin Red S assay, and Alkaline phosphatase (ALP) activity of the seed of Hei Chou ethanol extract was investigated to confirm the osteoblastic effect. The result was observed that the seed ethanol extract enhances a significant induction of ALP activity, alizarin red S assay when differentiated osteogenic from MC3T3-E1 cell. In conclusion, Hei Chou extracts increased ALP activity, alizarin red S assay activity for bone formation. These results suggest that Hei Chou acts a successful drug for osteogenic differentiation in vitro and has the potential for use as a therapeutic agent for bone formation.
Keywords :
biomedical materials; bone; botany; cellular biophysics; drugs; enzymes; molecular biophysics; Hei Chou ethanol extract concentrations; MC3T3-E1 cell; MC3T3-E1 cell calcification; alizarin red S assay; alkaline phosphatase activity; bone formation; osteoblastic effect; osteogenic cell; osteogenic differentiation in vitro; therapeutic agent; Biomembranes; Bones; Diseases; Drugs; Ethanol; In vitro; Mineralization; Calcification; Hei Chou; MC3T3-E1 Cell; Osteoblast; Pharbitis nil Choisy; component;
Conference_Titel :
Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on
Conference_Location :
Yantai
Print_ISBN :
978-1-4244-6495-1
DOI :
10.1109/BMEI.2010.5639494
