Title :
Iso-thermal DNA reactions and assays in microfabricated capillary electrophoresis systems
Author :
Tang, Thompson ; Ocvirk, Gregor ; Harrison, D. Jed
Author_Institution :
Dept. of Chem., Alberta Univ., Edmonton, Alta., Canada
Abstract :
The combination of microfluidic structures and electrokinetic separation and pumping on a glass chip, provides the capability for fast integrated, and more highly automated DNA assays. The Cycling Probe Technology reaction (CPT), used for signal enhancement of target DNA is an ideal candidate for integration on-chip. We show the application of the chip format for separations of CPT reaction products. Sensitivity with the chip format can be enhanced using epifluorescence confocal detection
Keywords :
DNA; biomedical equipment; biosensors; biotechnology; electrophoresis; micropumps; microsensors; separation; calibration curve; chip format; cycling probe technology reaction; electrokinetic separation; epifluorescence confocal detection; fast integrated assays; glass chip; highly automated DNA assays; hybridization assays; isothermal DNA reactions; microfabricated capillary electrophoresis systems; microfluidic structures; pumping; reaction products separation; signal enhancement; target DNA; Annealing; Biochemistry; Chemical technology; Chemistry; DNA; Electrokinetics; Glass; Microfluidics; Probes; Temperature;
Conference_Titel :
Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on
Conference_Location :
Chicago, IL
Print_ISBN :
0-7803-3829-4
DOI :
10.1109/SENSOR.1997.613702
