DocumentCode :
3143979
Title :
Derivation of oocyte-like cells from mouse embryonic stem cells
Author :
Hu, Yue ; Pan, Shaohui ; Wang, Jing ; Zhang, Shanshan ; Wang, Long ; Hua, Jinlian
Author_Institution :
Key Lab. for Reproductive, Physiol. & Embryo Biotechnol. of Agric. Minist. of China, Northwest A&F Univ., Yangling, China
Volume :
4
fYear :
2010
fDate :
16-18 Oct. 2010
Firstpage :
1659
Lastpage :
1663
Abstract :
In vitro derivation of oocytes from mouse embryonic stem cells (ESCs) would benefit the study of oogenesis. Here, we induced mouse ESCs to differentiate into oocyte-like cells by culturing adherent embryoid bodies (EBs) in different medium, including retinoic acid (RA) and follicular fluid. The extent of differentiation was evaluated through morphology and the expression of certain specific germ cell markers such as Oct4, Vasa, Scp3, Zp3, Gdf9 and Figla. As a result, EBs developed into ovary-like structures that contained oocyte-like cells showing expression of Oct4, Vasa, Gdf9, Figla and meiotic specific gene Scp3 and oocyte marker-Zp3. This study shows that RA and follicular fluid are beneficial for the derivation of germ cells and oocyte-like cells from mouse ES cells. The derivation of oocyte-like cells by our method offers an approach for studying oogenesis and regulation mechanism of mouse ESCs differentiation.
Keywords :
biological techniques; cellular biophysics; genetics; Figla; Gdf9; Oct4; Vasa; adherent embryoid bodies; cell follicular fluid; follicular fluid; meiotic specific gene Scp3; morphology; mouse embryonic stem cells; oocyte marker-Zp3; oocyte-like cells; oogenesis; retinoic acid; specific germ cell markers; Fluids; Humans; In vitro; Mice; Stem cells; Surface morphology; Suspensions; embryonic stem cells; follicular fluid; mouse; oocyte-like cells; retinoic acid (RA);
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on
Conference_Location :
Yantai
Print_ISBN :
978-1-4244-6495-1
Type :
conf
DOI :
10.1109/BMEI.2010.5639615
Filename :
5639615
Link To Document :
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