Effect of hesperidin extraction on cell proliferation and apoptosis of Alzheimer´s disease induced by Aβ25–35

Objective: To study the effect of hesperidin extracted from the peel of Gannan kumquat on Alzheimer´s disease and its possible mechanisms. Methods: Cellular model of Alzheimer´s disease was established using PC 12 cell induced by serum starvation method and treated by β-amyloid peptide 25-35 (Aβ_25-35). Effect of hesperidin on proliferation of PC12 cell induced by AP_25-35 was detected by the MTT method. Effect of hesperidin on apoptosis of PC12 cell induced by Aβ_25-35 was utilized by the acridine orange/ethidium bromide staining method. Results: PC12 cells stayed at Gο stage induced by serum starvation method in order to simulate the neuron in the state of terminal differentiation. After this, the PC cells induced by serum atarvation were treated by Aβ_25-35 to establish the cellular model of Alzheimer´s disease. After synchronized PC12 cells being pretreated with different concentrations of hesperidin (0-200 μmol/L) for 1 h, and then 30 μmol/L Aβ_25-35 for 24 h, it was found that the survival rate increased along with the increasing dosage of hesperidin (F = 995.8, P <;; 0.001); however, there was no significant difference between 100 μmol/L group and 200 μmol/L group (P > 0.05). After synchronized PC12 cells being pretreated with 100 μmol/L hesperidin for 1 h, and then 30 μmol/L Aβ_25-35 for 24h, the cell nuclei were gradually similar to the cell nuclei of control group by by acridine orange/ethidium bromide fluorescent staining.Conclusion: Hesperidin inhibited the cytotoxicity and apoptosis induced by Aβ_25-35 and had protection for neurocyte.