Title :
Quantitative Evaluation of Micro-motion of Vascular Endothelial Cells in Electrical Cell-substrate Impedance Sensing (ECIS) Method Using a Precision Mathematical Model
Author :
Goda, Noriko ; Yamamoto, Yoshitake ; Nakamura, Takao ; Kusuhara, T. ; Mohri, Satoshi ; Kataoka, Noriyuki ; Kajiya, Fumihiko
Author_Institution :
Okayama Univ. Med. Sch.
Abstract :
We constructed a precision mathematical model for quantitative evaluation of the micro-dynamics of cultured cells measured with ECIS (electrical cell-substrate impedance sensing) system that could separately estimate cell-to-cell distance and cell-to-substrate distance. For wide applications of this method, we constructed mathematical models describing for some kinds of cell and some kinds of confluent conditions. The mathematical models of these impedances could be fit well especially from 1 kHz to 10 kHz, which were most interesting frequency range for micro-dynamic analysis in ECIS methods
Keywords :
biological techniques; cellular biophysics; physiological models; 1 to 10 kHz; ECIS; cell culture; cell-to-cell distance; cell-to-substrate distance; electrical cell-substrate impedance sensing method; microdynamic analysis; micromotion; precision mathematical model; quantitative evaluation; vascular endothelial cell; Capacitance; Cells (biology); Electrodes; Equivalent circuits; Frequency; Gold; Immune system; Impedance; Mathematical model; Power system modeling; Cell culture; ECIS; Mathematical model;
Conference_Titel :
Microtechnologies in Medicine and Biology, 2006 International Conference on
Conference_Location :
Okinawa
Print_ISBN :
1-4244-0338-3
Electronic_ISBN :
1-4244-0338-3
DOI :
10.1109/MMB.2006.251480
