Evaluation of Lamination Micro Mixer for Micro Immunomagnetic Cell Sorter

Author

Inokuchi, H. ; Nagae, Kosuke ; Suzuki, Yuji ; Kasagi, Nobuhide ; Shikazono, Naoki

Author_Institution

Dept. of Mech. Eng., Tokyo Univ.

fYear

2006

fDate

9-12 May 2006

Firstpage

131

Lastpage

134

Abstract

A split-and-recombine lamination mixer for mu-immunomagnetic cell sorting system has been developed for extracting stem cells from peripheral blood. Cell suspension is mixed with antibody-coated magnetic beads, and target cells are labeled with antigen-antibody reaction. In this report, the mixer performance is evaluated quantitatively by using human umbilical vein endothelial cells (HUVEC). The number of magnetic beads attached to the target cell is evaluated in two different arrangements of the mixer units

Keywords

bioMEMS; biomedical engineering; blood vessels; cellular biophysics; antibody-coated magnetic beads; antigen-antibody reaction; cell suspension; human umbilical vein endothelial cells; microimmunomagnetic cell sorting system; peripheral blood; split-and-recombine lamination mixer; stem cells extraction; Biology; Blood; Cells (biology); Immune system; Lamination; Magnetic levitation; Magnetic separation; Mechanical engineering; Particle separators; Stem cells; Antigen-antibody reaction; Lamination micro mixer; Magnetic beads; Micro immunomagnetic cell sorter;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine and Biology, 2006 International Conference on

Conference_Location

Okinawa

Print_ISBN

1-4244-0338-3

Electronic_ISBN

1-4244-0338-3

Type

conf

DOI

10.1109/MMB.2006.251509

Filename

4281327