Title :
Evaluation of Lamination Micro Mixer for Micro Immunomagnetic Cell Sorter
Author :
Inokuchi, H. ; Nagae, Kosuke ; Suzuki, Yuji ; Kasagi, Nobuhide ; Shikazono, Naoki
Author_Institution :
Dept. of Mech. Eng., Tokyo Univ.
Abstract :
A split-and-recombine lamination mixer for mu-immunomagnetic cell sorting system has been developed for extracting stem cells from peripheral blood. Cell suspension is mixed with antibody-coated magnetic beads, and target cells are labeled with antigen-antibody reaction. In this report, the mixer performance is evaluated quantitatively by using human umbilical vein endothelial cells (HUVEC). The number of magnetic beads attached to the target cell is evaluated in two different arrangements of the mixer units
Keywords :
bioMEMS; biomedical engineering; blood vessels; cellular biophysics; antibody-coated magnetic beads; antigen-antibody reaction; cell suspension; human umbilical vein endothelial cells; microimmunomagnetic cell sorting system; peripheral blood; split-and-recombine lamination mixer; stem cells extraction; Biology; Blood; Cells (biology); Immune system; Lamination; Magnetic levitation; Magnetic separation; Mechanical engineering; Particle separators; Stem cells; Antigen-antibody reaction; Lamination micro mixer; Magnetic beads; Micro immunomagnetic cell sorter;
Conference_Titel :
Microtechnologies in Medicine and Biology, 2006 International Conference on
Conference_Location :
Okinawa
Print_ISBN :
1-4244-0338-3
Electronic_ISBN :
1-4244-0338-3
DOI :
10.1109/MMB.2006.251509
