Title :
Behaviour of Human Umbilical Vein Endothelial Cells (HUVEC) Cultivated in Microfluidic channels
Author :
Mulder, Patty P M F A ; Molema, Grietje ; Koster, Sander ; Van Der Linden, Heiko J. ; Verpoorte, Elisabeth
Author_Institution :
Res. Inst. of Pharmacy, Groningen Univ.
Abstract :
Our long-term goal is to develop advanced tools for cell studies and analysis based on microfluidic systems. In this paper, we report on endothelial cell cultivation in microchannels and 96-well tissue plates, and compare cell phenotype and cellular status in the two environments This was done under both pro-inflammatory conditions (cell stimulation in the presence of the cytokine, Tumour Necrosis Factor, TNFalpha) and normal (medium only) conditions. In addition, we considered the behaviour of cells in a pro-inflammatory environment in the presence of an anti-inflammatory drug
Keywords :
bioMEMS; cellular biophysics; drugs; microfluidics; molecular biophysics; proteins; HUVEC; antiinflammatory drug; cell phenotype; cell stimulation; cytokine; endothelial cell cultivation; human umbilical vein endothelial cells; microfluidic channel; pro-inflammatory conditions; tissue plates; tumour necrosis factor; Cells (biology); Drugs; Humans; Microchannel; Microfluidics; Pharmaceuticals; RNA; Reservoirs; USA Councils; Veins; HUVEC; cultivation; endothelial cells; microchannel; quantitative real-time reverse transcription PCR (RT-PCR);
Conference_Titel :
Microtechnologies in Medicine and Biology, 2006 International Conference on
Conference_Location :
Okinawa
Print_ISBN :
1-4244-0338-3
Electronic_ISBN :
1-4244-0338-3
DOI :
10.1109/MMB.2006.251515
