Title :
Dielectrophoresis, cell culture, and Electrical Impedance Spectroscopy Applied to Adherent Cells in a Single Biochip
Author :
Lennon, Erwan ; Ostrovidov, Serge ; Senez, Vincent ; Fujii, Teruo
Author_Institution :
CIRMM-IIS, Tokyo Univ.
Abstract :
In this work, we present a device that performs the 4 following functions: fluidic handling of biological cells suspension, electrical manipulation of cells by dielectrophoresis, cell culture and electrical impedance detection of adherent cells. Microfabrication process of the device, a hybrid glass/metal/PDMS structure, is detailed. Dielectrophoretic manipulation, culture and cell detection have been performed in a single device using human hepatocellular liver carcinoma cell. Results of broadband (10-107 Hz) electrical impedance spectroscopy (EIS) measurement performed on human cancerous cells are exposed
Keywords :
bioMEMS; biochemistry; bioelectric phenomena; biomedical measurement; cancer; cellular biophysics; electric impedance measurement; electrophoresis; liver; microfluidics; molecular biophysics; 10 to 107 Hz; adherent cells; biological cell suspension; broadband electrical impedance spectroscopy; cell culture; cell detection; dielectrophoretic manipulation; electrical manipulation; human hepatocellular liver carcinoma cell; hybrid glass-metal-PDMS structure; microfabrication process; microfluidics; single biochip; Anthropometry; Biological cells; Cells (biology); Dielectrophoresis; Electric variables measurement; Electrochemical impedance spectroscopy; Glass; Humans; Impedance measurement; Liver;
Conference_Titel :
Microtechnologies in Medicine and Biology, 2006 International Conference on
Conference_Location :
Okinawa
Print_ISBN :
1-4244-0337-5
Electronic_ISBN :
1-4244-0338-3
DOI :
10.1109/MMB.2006.251518
