Dielectrophoresis, cell culture, and Electrical Impedance Spectroscopy Applied to Adherent Cells in a Single Biochip

Author

Lennon, Erwan ; Ostrovidov, Serge ; Senez, Vincent ; Fujii, Teruo

Author_Institution

CIRMM-IIS, Tokyo Univ.

fYear

2006

fDate

9-12 May 2006

Firstpage

165

Lastpage

168

Abstract

In this work, we present a device that performs the 4 following functions: fluidic handling of biological cells suspension, electrical manipulation of cells by dielectrophoresis, cell culture and electrical impedance detection of adherent cells. Microfabrication process of the device, a hybrid glass/metal/PDMS structure, is detailed. Dielectrophoretic manipulation, culture and cell detection have been performed in a single device using human hepatocellular liver carcinoma cell. Results of broadband (10-10⁷ Hz) electrical impedance spectroscopy (EIS) measurement performed on human cancerous cells are exposed

Keywords

bioMEMS; biochemistry; bioelectric phenomena; biomedical measurement; cancer; cellular biophysics; electric impedance measurement; electrophoresis; liver; microfluidics; molecular biophysics; 10 to 10⁷ Hz; adherent cells; biological cell suspension; broadband electrical impedance spectroscopy; cell culture; cell detection; dielectrophoretic manipulation; electrical manipulation; human hepatocellular liver carcinoma cell; hybrid glass-metal-PDMS structure; microfabrication process; microfluidics; single biochip; Anthropometry; Biological cells; Cells (biology); Dielectrophoresis; Electric variables measurement; Electrochemical impedance spectroscopy; Glass; Humans; Impedance measurement; Liver;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine and Biology, 2006 International Conference on

Conference_Location

Okinawa

Print_ISBN

1-4244-0337-5

Electronic_ISBN

1-4244-0338-3

Type

conf

DOI

10.1109/MMB.2006.251518

Filename

4281336