Title :
Development and Evaluation of the Integrated In Situ Analyzer for Gene-"IISA-Gene" for Microbiology in Extreme Environments
Author :
Fukuba, Tatsuhiro ; Fujii, Teruo
Author_Institution :
Inst. of Ind. Sci., Tokyo Univ.
Abstract :
In this study an integrated in situ analyzer system for gene analysis (IISA-Gene) is developed to perform gene analysis of microorganisms inhabiting in extreme environments such as deep-sea or deep subsurface. Microfabricated chips made of PDMS and glass is employed to achieve a totally miniaturized and integrated system. Bacterial cell lysis, DNA purification, PCR amplification and amplified fragment detection can be performed with IISA-Gene system including miniaturized pumping and optical detection device. The IISA-Gene developed in this study was evaluated and operated in real deep-sea environment
Keywords :
DNA; bioMEMS; biosensors; cellular biophysics; microorganisms; microsensors; oceanographic techniques; optical sensors; seawater; DNA purification; IISA-Gene system; PCR amplification; PDMS substrate; bacterial cell lysis; chip microfabrication; deep subsurface; deep-sea; fragment detection; gene analysis; glass substrate; integrated in situ analyzer; microbiology; optical detection device; DNA; Glass; Microorganisms; Optical detectors; Optical devices; Optical pumping; Performance analysis; Purification; Stimulated emission; Ultraviolet sources; DNA purfication; Gene analysis; IISA-Gene; PCR; in-situ analysis;
Conference_Titel :
Microtechnologies in Medicine and Biology, 2006 International Conference on
Conference_Location :
Okinawa
Print_ISBN :
1-4244-0338-3
Electronic_ISBN :
1-4244-0338-3
DOI :
10.1109/MMB.2006.251523
