DocumentCode
3146509
Title
A Silicon-Based Single-Cell Electroporation Microchip for Gene Transfer
Author
Cho, Youngkyu ; Pioufle, Brno Le ; Takama, N. ; Kim, Bumki
Author_Institution
CIRMM-IIS, Tokyo Univ.
fYear
2006
fDate
9-12 May 2006
Firstpage
195
Lastpage
197
Abstract
In our contribution, we present the fabrication of electroporation microchip in detail. The practical experiments of single-cell electroporation with our fabricated microchip will be carried out. Electroporation test efficiency and cell viability tests will be provided. This device enables to reduce the size of samples and thus the use of small amount of reagents. It may also permit to avoid cell separation (transfected cells versus non transfected cells) encountered when traditional bulk electroporation is held
Keywords
bioMEMS; biochemistry; cellular biophysics; genetics; lab-on-a-chip; patient treatment; silicon; Si; bulk electroporation; cell viability tests; electroporation test efficiency; gene therapy; gene transfer; silicon; single-cell electroporation microchip fabrication; Cells (biology); DNA; Electrodes; Fabrication; Low voltage; Microchannel; Microelectrodes; Polymers; Scanning electron microscopy; Testing; Gene transfer; Microchip; Single-cell electroporation;
fLanguage
English
Publisher
ieee
Conference_Titel
Microtechnologies in Medicine and Biology, 2006 International Conference on
Conference_Location
Okinawa
Print_ISBN
1-4244-0338-3
Electronic_ISBN
1-4244-0338-3
Type
conf
DOI
10.1109/MMB.2006.251526
Filename
4281344
Link To Document