Title :
A Silicon-Based Single-Cell Electroporation Microchip for Gene Transfer
Author :
Cho, Youngkyu ; Pioufle, Brno Le ; Takama, N. ; Kim, Bumki
Author_Institution :
CIRMM-IIS, Tokyo Univ.
Abstract :
In our contribution, we present the fabrication of electroporation microchip in detail. The practical experiments of single-cell electroporation with our fabricated microchip will be carried out. Electroporation test efficiency and cell viability tests will be provided. This device enables to reduce the size of samples and thus the use of small amount of reagents. It may also permit to avoid cell separation (transfected cells versus non transfected cells) encountered when traditional bulk electroporation is held
Keywords :
bioMEMS; biochemistry; cellular biophysics; genetics; lab-on-a-chip; patient treatment; silicon; Si; bulk electroporation; cell viability tests; electroporation test efficiency; gene therapy; gene transfer; silicon; single-cell electroporation microchip fabrication; Cells (biology); DNA; Electrodes; Fabrication; Low voltage; Microchannel; Microelectrodes; Polymers; Scanning electron microscopy; Testing; Gene transfer; Microchip; Single-cell electroporation;
Conference_Titel :
Microtechnologies in Medicine and Biology, 2006 International Conference on
Conference_Location :
Okinawa
Print_ISBN :
1-4244-0338-3
Electronic_ISBN :
1-4244-0338-3
DOI :
10.1109/MMB.2006.251526
