Title :
Development of in situ ATP quantitative analysis system "IISA-ATP"
Author :
Fukuzawa, Noriyuki ; Fukuba, Tatsuhiro ; Fujii, Teruo
Author_Institution :
Inst. of Ind. Sci., Tokyo Univ.
Abstract :
An integrated in situ analyzer for ATP concentration (IISA-ATP) has been developed in this study. Concept of IISA-ATP that has function for cell lysis, extracellular ATP elimination, ATP quantification with luciferin-luciferase reaction and in situ calibration is proposed in this study. A simplified prototype of PDMS based microfluidic chip that has a coiled microchannel pattern for optical detection is developed with micro fabrication technique. As a result of evaluation of the prototype, more than 10-10 M range of ATP was successfully detected
Keywords :
bioMEMS; biochemistry; biological techniques; bioluminescence; cellular biophysics; chemical analysis; chemical variables measurement; ecology; enzymes; microchannel flow; microorganisms; molecular biophysics; seawater; ATP concentration analysis; ATP quantification; IISA-ATP; cell lysis; coiled microchannel pattern; extracellular ATP elimination; in situ ATP quantitative analysis system; in situ calibration; luciferin-luciferase reaction; marine ecosystems; microbial cells; microfabrication technique; microfluidic chip; optical detection; polydimethylsiloxane; seawater samples; Biological materials; Calibration; Ecosystems; Extracellular; Microfluidics; Optical detectors; Optical materials; Pollution measurement; Prototypes; Sea measurements; ATP; IISA-ATP; Luciferin-Luciferase reaction; PDMS; in-situ analysis;
Conference_Titel :
Microtechnologies in Medicine and Biology, 2006 International Conference on
Conference_Location :
Okinawa
Print_ISBN :
1-4244-0338-3
Electronic_ISBN :
1-4244-0338-3
DOI :
10.1109/MMB.2006.251544
