Effect of Oxygen Level on Efficiencies of Metabolic Fluxes in Klebsiella pneumoniae

Control-effective flux(CEF) analysis is a useful tool in assessing a metabolism. In this work, a model that utilizes existing knowledge of oxygen and redox sensing/regulatory system to assist elementary flux modes (EFMs), has been developed and CEF analysis was performed to analyze the influence of oxygen level on the transcriptional regulation in Klebsiella pneumoniae. It was found that the efficiency of reductive pathway was higher than oxidative pathway under anaerobic condition and reversed under aerobic condition. During the transition from anaerobic to aerobic conditions, the efficiencies of oxidative pathway in tricarboxylic acid and pentose phosphate pathway increased, and that the efficiencies of PDOR and other fermentative product pathway decreased.