مرکز منطقه ای اطلاع رساني علوم و فناوري - Effect of light irradiation and immobilization of carbon nanotubes on kinetic parameters corresponding to Michaelis — Menten equation, for NADH sensors based on phenothiazine graphite modified electrodes

DocumentCode :

3166373

Title :

Effect of light irradiation and immobilization of carbon nanotubes on kinetic parameters corresponding to Michaelis — Menten equation, for NADH sensors based on phenothiazine graphite modified electrodes

Author :

Ungurecan, M.-L. ; Gligor, D.-M.

Author_Institution :

Chem. Dept., Tech. Univ. of Cluj-Napoca, Cluj-Napoca, Romania

Volume :

fYear :

2010

fDate :

28-30 May 2010

Firstpage :

Lastpage :

Abstract :

The presence of light irradiation and immobilization of carbon nanotubes on graphite electrodes modified with polyphenothiazine formaldehyde (PPF) and bis-phenothiazin-3-yl methane (BPhM) leads to an increasing of sensitivity for NADH detection (calculated as I_max/K_M ratio). The paper presents the analogical modelling and numerical simulation for fitting the Michaelis-Menten equation to experimental data by a regression analysis. The program determines the theoretical kinetic parameters: K_M and I_max and they are compared with the values of the same parameters experimentally determined.

Keywords :

amperometric sensors; biochemistry; biological techniques; carbon nanotubes; catalysis; electrodes; enzymes; graphite; molecular biophysics; nanosensors; nanotube devices; oxidation; photoelectrochemistry; radiation effects; reaction kinetics theory; reduction (chemical); regression analysis; C; Michaelis-Menten equation; NADH detection; NADH sensor; amperometric detection; analogical modelling; bis-phenothiazin-3-yl methane; carbon nanotube immobilization; coenzyme; electrocatalytic oxidation; kinetic parameters; light irradiation; nicotinamide adenine dinucleotide; numerical simulation; phenothiazine modified graphite electrodes; photoelectrocatalytic oxidation; polyphenothiazine formaldehyde; redox mediators; regression analysis; sensitivity; Carbon nanotubes; Chemical technology; Electrodes; Electrons; Equations; Kinetic theory; Numerical models; Numerical simulation; Oxidation; Polymer films; Michaelis-Menten equation; NADH; Phenothiazine derivatives; carbon nanotubes; modified graphite electrodes; numerical modelling; simulation;

fLanguage :

English

Publisher :

ieee

Conference_Titel :

Automation Quality and Testing Robotics (AQTR), 2010 IEEE International Conference on

Conference_Location :

Cluj-Napoca

Print_ISBN :

978-1-4244-6724-2

Type :

conf

DOI :

10.1109/AQTR.2010.5520775

Filename :

5520775

Link To Document :

https://search.ricest.ac.ir/dl/search/defaultta.aspx?DTC=49&DC=3166373