Title :
Selection of fungus with high ability of cellulase activity production using UV mutagenesis
Author :
Liu, Jia ; Feng, Yujie ; Yu, Yanlin ; Zhou, Xiangtong ; He, Weihua
Author_Institution :
State Key Lab. of Urban Water Resource & Environ. (SKLUWRE), Harbin Inst. of Technol. (HIT), Harbin, China
Abstract :
Mutation breeding was carried out on Aspergillus nidulans. to obtain strains with high cellulase activity. One mutant strain, U6, with high cellulase activity was obtained through primary screening and secondary screening, which used Congo red medium to compare the hydrolyzed ring and liquid fermentation to compare the enzyme activity, respectively. The enzyme activity of the obtained mutant rose from 39.60 U/ml to 156.79 U/ml, 3.96 times higher than that of original strains.
Keywords :
biochemistry; enzymes; fermentation; microorganisms; molecular biophysics; Aspergillus nidulans; Congo red medium; UV mutagenesis; cellulase activity; enzyme activity; fungus; hydrolyzed ring; liquid fermentation; mutation breeding; Biochemistry; Biomass; Biotechnology; Degradation; Strain; Sugar; Aspergillus nidulans; breeding; cellulase; mutagenesis;
Conference_Titel :
Consumer Electronics, Communications and Networks (CECNet), 2011 International Conference on
Conference_Location :
XianNing
Print_ISBN :
978-1-61284-458-9
DOI :
10.1109/CECNET.2011.5769254
