Title :
Micromachined cellular characterization system for studying the biomechanics of individual cells
Author :
Ayliffe, H. Edward ; Rabbitt, Richard D. ; Tresco, Patrick A. ; Frazier, A. Bruno
Author_Institution :
Dept. of Bioeng., Utah Univ., Salt Lake City, UT, USA
Abstract :
Micromachining technologies have enabled the development of microchannel devices for studying the migration behavior of individual living cells in stable chemotactant gradients. A micromachined three-dimensional system for cellular characterization was designed, fabricated, and tested. Human leukocytes were characterized as they crawled on multiple surfaces toward a distant chemotactic source. Excellent optical clarity was maintained by constructing the microchannels from polished glass and polyimide. Results of the system design and various environmental effects on cellular morphology and motility are presented
Keywords :
biological techniques; biomechanics; blood; cell motility; cellular biophysics; micromachining; cellular migration behavior; cellular morphology; distant chemotactic source; environmental effects; human leukocytes; individual cells biomechanics study; microchannels; micromachined cellular characterization system; optical clarity; polished glass; polyimide; stable chemotactant gradients; Adhesives; Biomechanics; Biomedical optical imaging; Chemistry; Glass; Microchannel; Micromachining; Surface morphology; Surface treatment; White blood cells;
Conference_Titel :
Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on
Conference_Location :
Chicago, IL
Print_ISBN :
0-7803-3829-4
DOI :
10.1109/SENSOR.1997.635476
