Surface plasmon resonance as a tool for monitoring multiple biomolecular interactions

Surface plasmon resonance (SPR) technique has become a powerful tool for characterizing and quantifying biomolecular interactions. The measurement of the reflectivity changes allows monitoring the adsorption of biomolecules into patterned molecular arrays. For example, SPRI can be used for studying the specific adsorption of biomolecules (antibodies, proteins, DNA) onto DNA and protein arrays. Real time images can be recorded as the binding reactions occur and the change in reflectivity, proportional to the amount of molecules locally adsorbed onto the surface, can be measured. The SPRI technique is very sensitive and can be used both in-situ and ex-situ configurations to analyze in real time hybridization of label-free molecules to metal surface. The analysis is performed on the 2D image of the metal surface made from a gold film deposition.