Title :
Detection of 100 spots parallel DNA:DNA interactions on polymer-functionalized surfaces using surface plasmon resonance imaging
Author :
Bassil, Nathalie ; Maillart, Emmanuel ; Canva, Michael ; Lévy, Yves ; Millot, Marie-Claude ; Pissard, Serge ; Narw, Rémy ; Goossens, Michel
Author_Institution :
Lab. Charles Fabry de l´´lnst. d´´Opt., Univ. Paris XI, Orsay, France
Abstract :
Surface plasmon resonance is a method well-known for its sensibility to refractive index variations at a metal-dielectric interface. Surface plasmon imaging is an extension of this method. It allows the local study of refractive index variations resulting from interfacial modifications on a metallic surface. This technique is suitable to monitor in parallel, in real time and without any label hundreds of molecular interactions. The study of DNA:DNA hybridization is one of its applications.
Keywords :
DNA; biomedical optical imaging; optical polymers; refractive index; surface plasmon resonance; DNA interactions; DNA: DNA hybridization; interfacial modifications; metal-dielectric interface; metallic surface; molecular interactions; polymer-functionalized surface; refractive index variation; surface plasmon resonance imaging; DNA; Dielectrics; Genetic mutations; Monitoring; Plasmons; Polymers; Probes; Refractive index; Resonance; Sequences;
Conference_Titel :
Lasers and Electro-Optics Europe, 2003. CLEO/Europe. 2003 Conference on
Print_ISBN :
0-7803-7734-6
DOI :
10.1109/CLEOE.2003.1313787
