Title :
Metallic tip enhanced fluorescence for DNA replication monitoring
Author :
Shih-Chung Wei ; Tsung-Liang Chuang ; Kung-Bin Sung ; Hui-Hsin Lu ; Chii-Wann Lin
Author_Institution :
Inst. of Biomed. Electron. & Bioinf., Nat. Taiwan Univ., Taipei, Taiwan
Abstract :
We have successfully performed localized loop-mediated isothermal reactions of hepatitis B virus (HBV) and hepatitis C virus (HCV) on the apex (50~100 nm) of metallic tips coated with Bst polymerases. The SYBR green molecules binding to the new formed HBV DNA inside the optical near fields were excited by two-photon fluorescence microscopy, and directly imaged in far field. Another reporter primer is used for HCV replication detection. Preliminary results are presented in this manuscript.
Keywords :
DNA; enzymes; fluorescence; microorganisms; molecular biophysics; molecular configurations; optical microscopy; two-photon spectra; Bst polymerases; DNA replication monitoring; HCV replication detection; SYBR green molecules binding; hepatitis B virus; hepatitis C virus; localized loop-mediated isothermal reactions; metallic tip enhanced fluorescence; optical near fields; size 50 nm to 100 nm; two-photon fluorescence microscopy; Biomedical optical imaging; DNA; Fluorescence; Gold; Isothermal processes; Microscopy; Polymers;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2013 35th Annual International Conference of the IEEE
Conference_Location :
Osaka
DOI :
10.1109/EMBC.2013.6609543
