Title :
Low-repetition rate CARS-microscopy: biomedical applications
Author :
Thogersen, J. ; Jorgensen, P. ; Keiding, S.R. ; Nilsson, H.
Author_Institution :
University of Aarhus
Abstract :
We have constructed a microscope based on coherent anti-Stokes Raman scattering using a regeneratively amplified femtosecond laser operating at 1 kHz. So far CARS-microscopes have primarily been constructed using high repetition rate laser systems operating above 1 MHz. The present microscope demonstrates very high spatial resolution with a minimum of thermal load on the samples. Preliminary results of CARS- microscopy on cornea-samples and cellular structures are presented.
Keywords :
cellular biophysics; coherent antiStokes Raman scattering; eye; high-speed optical techniques; laser applications in medicine; optical microscopy; 1 kHz; CARS-microscopy; amplified femtosecond laser; biomedical applications; cellular structures; coherent anti-Stokes Raman scattering; cornea-samples; high repetition rate laser systems; microscope; spatial resolution; thermal load; Laser tuning; Microscopy; Optical amplifiers; Optical filters; Optical pulse generation; Optical pulses; Pulse amplifiers; Thermal loading; Tunable circuits and devices; Ultrafast optics;
Conference_Titel :
Quantum Electronics Conference, 2003. EQEC '03. European
Print_ISBN :
0-7803-7733-8
DOI :
10.1109/EQEC.2003.1314029
