Application of fluorescent PSF for 3D reconstruction of absorbing structure using slab transillumination images

Author

Trung Nghia Tran ; Namita, T. ; Kato, Yu ; Shimizu, Kazuo

Author_Institution

Grad. Sch. of Inf. Sci. & Technol., Hokkaido Univ., Sapporo, Japan

fYear

2013

fDate

3-7 July 2013

Firstpage

2644

Lastpage

2647

Abstract

In transillumination imaging of an animal body using near-infrared light, the image is blurred due to the strong scattering in the tissue. We have devised the depth-dependent point spread function (PSF) to suppress the scattering effect in fluorescent imaging. In this study, we applied this principle and developed a technique to reconstruct the absorbing structure in turbid medium without using fluorescent material. In experiments, the feasibility and effectiveness of the proposed technique were verified.

Keywords

biological tissues; biomedical optical imaging; fluorescence; image reconstruction; infrared imaging; medical image processing; optical transfer function; turbidimetry; 3D reconstruction; PSF; fluorescent PSF; fluorescent imaging; near-infrared light; point spread function; slab transillumination images; turbid medium; Animals; Deconvolution; Fluorescence; Image reconstruction; Imaging; Scattering; Three-dimensional displays;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2013 35th Annual International Conference of the IEEE

Conference_Location

Osaka

ISSN

1557-170X

Type

conf

DOI

10.1109/EMBC.2013.6610083

Filename

6610083