Determination of l-menthol in Qingxuanwan by callipary GC

Author

Sun, Cong ; Han, Yechao ; Wu, Qiang

Author_Institution

Traditional Med., Changchun Univ. of Chinese, Changchun, China

Volume

2

fYear

2012

fDate

3-5 Aug. 2012

Firstpage

816

Lastpage

818

Abstract

[Purpose] To develop an GC method for content determination of l-menthol in Qingxuanwan. [Methods] The sample was determinated by GC after being extracted by ethanol with ultrasonic extractor. The GC-FID experiments were performed on an Aglient 6890N GC equipped with flame ionization detector (FID). DB-WAX column (30 m × 0.25 mm, 0.25μm) was used. The injector, column and detecto rtemperatures were 250°C, 160°C and 250°Crespectively. The samples were injected by split mode. The ratio of split was 5:1. The flow rate was 1.0 ml/min. The injection volume was 2 μl. [Results] The average recovery was 98.0% and RSD was 0.7%(n=6). [Conclusion] The method is proved to be sensitive and accurate, and can be used to control the quality of Qingxuanwan.

Keywords

chemical reactors; chromatography; drugs; pharmaceutical technology; quality control; ultrasonic devices; Aglient 6890N GC; DB-WAX column; GC-FID; L-menthol content determination; Qingxuanwan; callipary GC method; ethanol; extraction; flame ionization detector; gas chromatography; injection volume; quality control; temperature 160 degC; temperature 250 degC; ultrasonic extractor; Companies; Education; Nitrogen; USA Councils; FID; Qingxuanwan; callipary GC; determination; l-menthol;

fLanguage

English

Publisher

ieee

Conference_Titel

Information Technology in Medicine and Education (ITME), 2012 International Symposium on

Conference_Location

Hokodate, Hokkaido

Print_ISBN

978-1-4673-2109-9

Type

conf

DOI

10.1109/ITiME.2012.6291428

Filename

6291428